Earthworm Group (Elphinstone College)
So Ritik and me are working on the simple model organism, Earthworms (species: Eisenia fetida)
Long term objective:
To study nerve cord regeneration in earthworms. So we do this particular assay called Rapid Escape Response (RER). So in this assay, we take an earthworm and two Testubes one contains distilled water and the other one contains 200mM solution of NaCl and we put a timer for 2 minutes. And we introduce the earthworm first in distilled water testtube.
And then if the earthworm does not come out in 2min. Then we introduce it in the salt solution.
So our expectations are that the earthworm will not come out of the distilled water TT (testube). And it will come out of the NaCl soln TT.
Since distilled water does not have any ions it does not irritate the earthworm but since NaCl soln has has Sodium and chloride ions it irritates the earthworm hence the earthworm
comes out under 2 mins. So we have done many rounds of RER It mainly helps us to check whether the earthworm’s nerve cord is intact or not for further experiments
So after the RER we gave lesion on 17th of April
- 4 earthworms for dorsal cut
- 4 earthworms for ventral cut (control setup)
- 4 earthworms for normal earthworms (control setup)
On giving lesion we see that the ventral cut earthworms are not able to climb out of the NaCl TT. But after 48hrs the earthworms regenerate.