Establishing moina culture in smt.C.H.M.College 2

Yes it will work more better higher the concentration of moina can easily be transferred with dropper or pippet

I have something else in my mind. Leave aside the collection problem through a pipette or a dropper. Think about which container is likely to have more oxygen?

Oxygen in water comes by the simple process of diffusion so diffusion of oxygen is directly proportional to the surface area of the water so grater the surface area higher the amount of oxygen dissolved in water

3 Likes

The reference is our seniors’ thesis.
As per my knowledge, Anacardic Acid an anticancer agent inhibits HAT.
Haemoglobin remains suspended where??
Can you please elaborate the 4th question?

Haemoglobin is a protein which helps in gaseous transport in case of higher organisms in which rbcs are present in blood in them haemoglobin associates with rbcs and circulate in the body and in the case of moina it have haemolymph so what happens with haemoglobin after development

1 Like

Any relevant reference?

Generally, Hb is formed when there is an oxygen deficit. The Hb content of Daphnia decreases before a molt, but increases in the ovaries at the same time (Fox et al., 1949).

https://www.sciencedirect.com/topics/agricultural-and-biological-sciences/moina

In this article in respiration part relationship of oxygen and haemoglobin is mentioned

2 Likes

2019-08-16T18:30:00Z
Today i tried to take some good photographs of moina under microscope in CUBE lab HBCSE
so on 10/08/2019 I’ve replied on a question in which I’ve mentioned that ,
About male and female I’ve only idea that females have comparitively larger size than males and can be easily identified with their brood pouch

Today I’ve some photos to share with all so what large size and small size deals with is

Male



Female

First 3 image represents male while next 3 female
From above image I can conclude that
By gross structure
1)male have spindle shape
2) female have circularshape

So other than this 2 criteria ihad mentioned about brood pouch
In 3 images of females we can observe on dorsal side or on the back region we can clearly se there are some moina in the brood pouch we can even observe there eyes ie eyes of progeny’s in brood pouch

So to see or to observe the comparitive size of moina we can refer the below images

Video

In this video there are 2 female 1at centre 1 at left hand side top corner
And 1 male moving around female at center


3 female at the center and 5 male moving around them

6 Likes

Which species of Moina have you observed here?

Currently idont have idea about the species which I’m studying but with reference to a reply of @drishtantmkawale if the species we are dealing with Moina macrocopa then the document attached below states what Moina macrocopa organisms is and how it looks like now the point is we need to confirm it
Elmoor- loureiroRBBnewreportofMoinamacrocopainSouthAmerica.pdf (875.2 KB)
Document

[Uploading: VID-20190817-WA0011.mp4…]
Moina giving birth to new young progeny.

2 Likes

@Siddhesh can you elaborate some salient characters of Moina macrocopa?

How is it different from any other species of Moina?

Moina species key identification:
1a.A simple distal tooth (next to the claw) in the row of teeth along the dorsal margin of the postabdomen; western N.A. saline lakes Moina hutchinsoni
1b.A double (bifid) distal tooth (next to the claw) in the row of teeth along the dorsal margin of the postabdomen 2
2a (1b).A row of teeth along the side of the setae on the last and penultimate segments of the first thoracic leg; ephippium with two eggs; north temperate, coast to coast Macrocopa.
2b.Feathery setules along the side of the setae on the last and penultimate segments of the first thoracic leg; ephippium with one or two eggs 3
3a (2b)Long hairs on head and carapace 4
3b.Hairs completely absent from head and carapace 5
4a (3a).Never over 1.2 mm long, one egg per ephippium; an uncommon species found from midwestern and southeastern United States and west into Mexico M. affinis
4b.Some adults longer than 1.2 mm; two eggs per ephippium; southwestern United States to Argentina M. wierzejski
5a (3b).Length less than 1.2 mm, one egg per ephippium; temporary ponds to large lakes in the north temperate zone, coast to coast and south to Paraguay; from temporary ponds to large lakes Moina micrura. The N.A. form is widely used in ecotoxicology and aquaculture.
]5b.Some adults longer than 1.2 mm; two eggs per ephippium; one population known from southern California M. brachycephala

Reference:Cladocera and Other Branchiopoda
Stanley L. Dodson, D. Christopher Rogers, in Ecology and Classification of North American Freshwater Invertebrates (Third Edition), 2010
https://doi.org/10.1016/B978-0-12-374855-3.00020-0

4 Likes

@akshita how does a moina look?
can you get some picture of Moina macrocopa and mark these salient characters in the picture?

2 Likes

It’s good message but you can make it better with supporting photographs @Akshitha

2019-08-18T18:30:00Z
So as I I’m working on moina so it is fascinating experience to observe moina changing it’s colour from red to colour less and vice versa so while experimenting igot red moina on 11th augast igot read moina

So that intense red colour igot on the day but I added water in it around 30 ml but proportionally didn’t increased amount of milk drops as feeding so now to compare the colour we can refer the image below
According to me this red colour is not intense as what I’ve shown in video recorded on 11th augast

White moina in culture

Red moina in culture

Compitative photo for moina colour

I’m reporting from my kitchen lab
Ulhas nagar
19.2215° N, 73.1645° E

2 Likes

Have you covered the glass bottles wont the moina suffocate if you cover it

How could moina will suffocate I’m unable to get the question

No moina will not suffocate because moina need oxygen nd in water Oxygen dissolved so they only need water food and if don’t cover the lid so many problem increased . So always cover the culture .

1 Like

But that bottle which you showed with red moinas on 11 august is different from the bottle you are showing today. Have you transferred moinas to some other container? Why is the count of moinas so less in the picture as compared to the video? @Siddhesh

1 Like