Moina Group Discussion

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5ug concentration


10ug concentration


50ug concentration

100ug concentration


500ug


Control 1


Control 2

All the observations were done under 10x magnification.

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CUBE Elphinstone College
Moina Group

Short term Objective: To Culture red moinas.

Long term objective: To study the effect of VPA in gene expression in Moinas.

Our VPA setup is prepared but then too we are maintaining a red moina culture.

Today’s observation: Bottle D1 has approximately 60-70 moinas with ~ 20 moinas with red colour and other having pale yellow colour and many having small size and the same was observed in replica bottle V1 with ~ 50-60 moinas.

Tomorrow’s expectations: 70-80 moinas in Bottle D1 and 60-70 moinas in Bottle V1 with ~20 having red colour.

On 27 August 2019, we prepared our VPA (HDAC Inhibitor - doesn’t allow red moinas to turn to colourless easily) setup. We took 200mg VPA tablets from a pharmacy and out of that a tablet was dissolved in 200ml of water to get 1mg/ml.
Then we decided to take 5 concentrations of VPA i.e. 5ug, 10ug, 50ug, 100ug and 500ug. We took large differences between the concentrations to study its effect at large concentrations. For example, we hypothesize that moinas in 500ug concentration will take more time to get Colourless.
The concentrations were prepared as follows:
5ug- 995ml Water + 5ml of 1mg/ml VPA.
10ug - 990ml water + 10ml of 1mg/ml and so on.
Then we added 5 Red moinas in each of the 12 test tubes (10 concentrations with replicas + 2 control) containing 10ml DC water.
We decided to feed them with 1:25 ( 1 drop of milk and 25 drops of water) dilution because for 250ml of water we add 1 drop of milk, for 25ml we add 1:10 dilution and for 10ml 1:25 dilution.

Today’s observation of the VPA setup : Not much change observed in the colour of Moinas.

Tomorrow’s expectations for the VPA setup : We expect a slight change in colour in the test tubes having concentrations.

Update about 6L Moina Tank Culture : There are about 150 moinas in the culture and almost all of them are Colourless.

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CUBE Elphinstone College
Moina Group

Objective: To study the effect of VPA in gene expression in Moinas.

Our VPA setup is prepared but then too we are maintaining a red moina culture.

Red Moina Culture started on 19/08/2019

Got red moinas by 26/08/2019

Made VPA setup on 27/08/2019

Today’s observation of red moina culture : Bottle D1 has approximately 100 moinas with ~ 40 moinas with red colour and others having pale yellow colour and many having small size and the same was observed in replica bottle V1 with ~ 70-80 moinas.

Tomorrow’s expectations : 120 moinas in D1 with ~ 60-70 red moinas and 100 moinas in V1 with ~ 40 red moinas

Yesterday’s and Today’s observation of VPA setup:

Tomorrow’s expectations for the VPA setup : We expect a slight change in colour in the test tubes having concentrations.

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What is epigenetic? @drishtantmkawale

Handling microscope properly could give you more better pics which will eventually help us too to see what exactly is happening to moinas, thus making your work simpler and letting people know what you want to show through these pics… Also I have seen chnge in these moinas, imean on 28 n on 29th both aren’t the same, if it could be same we could have easily spot the differences between today and tomorrow… Are you getting my point @drishtantmkawale. What if you only put one red moina in each setup and observe only that one moina daily, you could easily spot the differences if there will be any.

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We can put a single Moina, but if it is female, then it will reproduce and produce more moinas although of small size but same red colour! and finding a male is very rare because moinas reproduce asexually for majority of the times which would give female progeny and to reproduce sexually, again we need one male. But we are trying to find one.

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Objective : To culture moinas as of now and later study them (for future reference)

So today I, @Sjuday2527 @Spandan_b and Namrata from Elphinstone College prepared two 5L Moina Tank Cultures and two Moina Bottle Cultures.

Preparation of Tank Cultures:
Washed the tanks thoroughly with tap water and then DC water and then took 5L of DC water in the
tanks and added ~ 20 moinas in both the tanks feeding them with 5 drops of milk.

Expectations for tomorrow: ~ 30 moinas

Preparation of Bottle Cultures:
Two 1L bottles were washed thoroughly with tap water first and then DC water.
In one bottle, 250ml of DC water + 5 - 10 colourless moinas + 4 drops of milk. 4 drops of milk because we want to study the time period taken by the moinas to get red in colour in 4drops as we know that they take ~ a week to get red in 6 drops of milk.

Expectations for tomorrow: ~15 colourless moinas.

And in the other bottle, 750ml DC water + 3 drops of milk (1 drop of milk in 250ml DC water so 3 in 750ml) + ~ 10 colourless moinas.

Expectations for tomorrow: ~ 15 moinas.

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Epigenetics is the study of phenotypic (observable characteristics) changes in the organism without altering its DNA Sequence.

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Moina Group
@Spandan_b @Jyotiraditya
Yesterday at the causerie there was a question that do moinas survive in Distilled Water?

We had not tried that earlier so we tried it today.

Procedure for Moina culture in Distilled Water
We took 10ml of Distilled water in a clean test tube and added 5 colourless moinas from another bottle and we fed them with 1:25 dilution i.e. 1 drop of milk and 25 drop of distilled water (Why?). As normally we add 1 drop of milk in 250ml of Water → 1:10 dilution in 25ml of water→ 1:25 dilution in 10ml of water.

We hypothesize that the moinas won’t survive for much time as their environment (distilled water) is free from nutrients and minerals and as we humans too require essential minerals like Calcium (for blood clotting), Phosphorus (healthy teeth and bones) and many more, they too require them.

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@bivasnag we were discussing about this :point_up_2:

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CUBE Elphinstone College
Moina Group

Short term Objective: To have a pure Moina culture without rotifers or any other organisms

Long term objective: To study histone remodeling in moina via Haemoglobin protein expression through epigenetics

Immediate Objective: To culture moinas

Today, bottles D1, D2(replica), S1, M1(replica) were prepared by taking 250ml DC water in 4 bottles + 5 moinas in each + 1 drop in D1, D2 and 4 drops in S1 and M1.

Tomorrow’s expectations: ~5-10 moinas in all bottles.

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CUBE Elphinstone College
Moina Group

Short term Objective: To have a pure Moina culture without rotifers or any other organisms (Monoxenic culture - a culture containing one species growing in the presence of the other )

Long term objective: To study histone remodeling in moina via Haemoglobin protein expression through epigenetics

Immediate Objective: To culture moinas

On 9 September 2019, bottles D1, D2(replica), S1, M1(replica) were prepared by taking 250ml DC water in 4 bottles + 5 moinas in each + 1 drop in D1, D2 and 4 drops in S1 and M1.

Today’s observations:
D1 ~ 5 moinas
D2 ~ 10 moinas
M1 ~ 5 moinas
S1 ~ 20 moinas

Maybe the Moinas transferred in bottles D1 and M1 were small, so they would take time to reproduce.

Friday’s expectations:
D1 ~ 10 moinas
D2 ~ 20 moinas
M1 ~ 10 moinas
S1 ~ 30 moinas

These bottles are kept in our college lab.

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CUBE Elphinstone College
Moina Group

Short term Objective: To have a pure Moina culture without rotifers or any other organisms (Monoxenic culture)

Long term objective: To study histone remodeling in moina via Haemoglobin protein expression through epigenetics

Immediate Objective: To culture moinas

On 9 September 2019, bottles D1, D2(replica), S1, M1(replica) were prepared by taking 250ml DC water in 4 bottles + 5 moinas in each + 1 drop in D1, D2 and 4 drops in S1 and M1.

Today’s observations:
D1 ~ 7 moinas
D2 ~ 15 moinas
M1 ~ No moinas
S1 ~ 35 moinas

The moinas in Bottle M1 died so I added 5 moinas from bottle S1, so now there are ~ 30 moinas in S1

Tomorrow’s expectations:
D1 ~ 10 moinas
D2 ~ 20 moinas
M1 ~ 5-7 moinas
S1 ~ 45 moinas

These bottles are kept in our college lab

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CUBE Elphinstone College
Moina Group

Objective : To study the effect of distilled water on moinas.

Today 13 September 2019, we prepared 4 test tube A1, A2 (replica) , B1, B2 (replica) having Moinas in Distilled water.

Procedure:
We took 4 sterilized test tubes, added 10ml of Distilled Water in each test tube + 5 moinas in each test tube + 1:25 dilution of Milk ( 1 drop of milk + 25 drops of distilled water) in tube A1 and A2 only. The moinas in tube B1 and B2 were not fed with milk. Some Moinas were gravid

IMG_20190913_134524|690x388

Tomorrow’s expectations:
5-10 Moinas in A1 and A2 as nutrition is being provided to them but we also feel that the bacteria/microorganisms present in the milk too will need nutrition and they may not survive.

No moinas in B1 and B2.

How to check for Distilled water?
A. We took a pH paper and dipped it in the distilled water which we had… the pH was matching to 7 which is the ideal pH of Distilled Water. Some references mention the pH to be slightly acidic because it absorbs CO2 from the atmosphere.

Reference: What is the pH of Distilled Water? Alkaline or Acidic? – WaterDistillers.com

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CUBE Elphinstone College
Moina Group

Objective : To culture moinas in a Tank.

Today 13 September 2019, we prepared a 6L tank of Moinas.

Procedure:
A big tank with ~ 10L volume was taken.
In that 6L DC Water + ~ 20 colourless moinas + 10 drops of milk (it’s just a rough volume as the objective is just to culture moinas).

Tomorrow’s expectations:
20-30 moinas.

This tank culture is kept in our College Lab.

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CUBE Elphinstone College
Moina Group

Breaking news!!

The moinas in the four test A1, A2 (both with milk), B1 and B2 (both without milk) didn’t survive in Distilled Water whose pH was 7.


A1 (with milk)


A2 (with milk)


B1 (without milk)


B2 (without milk)

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CUBE Elphinstone College
Moina Group
@⁨Zahra Risalawala⁩
@Hammad Khan

Objective : To culture moinas in a Tank.

On 13 September 2019, we prepared a 6L tank of Moinas.

Procedure:
A big tank with ~ 10L volume was taken.
In that 6L DC Water + ~ 20 colourless moinas + 10 drops of milk (it’s just a rough volume as the objective is just to culture moinas). No specific calculations were done for adding 10 drops of milk.

Today’s observation: 60-70 colourless moinas

Monday’s expectations:
~100 - 120 colourless moinas

This tank culture is kept in our College Lab

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CUBE Elphinstone College
Moina Group

Short term Objective: To have a pure Moina culture without rotifers or any other organisms (Monoxenic culture)

Long term objective: To study histone remodeling in moina via Haemoglobin protein expression through epigenetics

Immediate Objective: To culture moinas

On 9 September 2019, bottles D1, D2(replica), S1, M1(replica) were prepared by taking 250ml DC water in 4 bottles + 5 moinas in each + 1 drop in D1, D2 and 4 drops in S1 and M1.

Today’s observations:
D1 ~ 5 moinas
D2 ~ 20 moinas
M1 ~ 5-7 moinas
S1 ~ 40-50 moinas

Monday’s expectations:
D1 ~ 10 moinas
D2 ~ 30 moinas
M1 ~ 10 -15 moinas
S1 ~ 60 moinas

These bottles are kept in our college lab.

2 Likes

CUBE Elphinstone College
Moina Group

Objective : To culture moinas in a Tank.

On 13 September 2019, we prepared a 6L tank of Moinas.

Procedure:
A big tank with ~ 10L volume was taken.
In that 6L DC Water + ~ 20 colourless moinas + 10 drops of milk (it’s just a rough volume as the objective is just to culture moinas so there is no specific calculation for adding 10 drops of milk).

Today’s observation: ~160 colourless moinas

Tomorrow’s expectations:
~ 200 moinas

This tank culture is kept in our College Lab.

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