Short term Objective: To have a pure Moina culture without rotifers or any other organisms (Monoxenic culture)
Long term objective: To study histone remodeling in moina via Haemoglobin protein expression through epigenetics
Immediate Objective: To culture moinas
On 9 September 2019, bottles D1, D2(replica), S1, M1(replica) were prepared by taking 250ml DC water in 4 bottles + 5 moinas in each + 1 drop in D1, D2 and 4 drops in S1 and M1.
For tomorrow, we are planning to change the water of these cultures and will provide the same conditions as earlier.
The existing water would-be checked for rotifer growth i.e. we are going to look for clumps if any…
The moinas would be transferred to the beaker, new DC water would be added in the bottle (the bottle would be washed) and then moinas would be transferred by dropper.
Short term Objective: To have a Monoxenic culture of Moinas
Long term objective: To study histone remodelling in Moina via Haemoglobin protein expression through epigenetics.
Immediate Objective: To culture moinas
On 9 September 2019, bottles D1, D2(replica), S1, M1(replica) were prepared by taking 250ml DC water in 4 bottles + 5 moinas in each + 1 drop-in D1, D2 and 4 drops in S1 and M1 and on 13 September 2019
Bottles S13 and S2 were prepared with the same protocol as above by adding 250ml DC Water + 10 Moinas + 1 drop of milk in each.
This is the Moina Growth Kinetics from 9 September 2019 to 19 September 2019.
I think when there is an increase in the Moina count (crowding) and when we feed the moinas, the condition for them becomes stress condition i.e. crowding + number of organisms through milk increase because of which the moinas have to compete for oxygen so their number decreases. I have observed this in my previous cultures but data is not available for them
In the bottles first. As the surface area in the tank is more, the moinas can wander everywhere, also the ammonia levels will also be less in the 6L tank culture as compared to 250ml bottle culture.
As @pratittodkar gave the reference, excess of ammonia can result in the death of the crustaceans.
Good question!
But, in my case I’m not feeding the moinas in the tank culture according to the protocol.
Protocol is 1 drop of milk in 250ml DC water for normoxic condition.
I’m feeding the tank culture with 10 drops of milk which should have been 24 drops of milk according to the protocol!
What if the protocol isn’t right and the Moinas actually thrive better in 1 drop/liter instead of 1 drop/250 ml? Are there any experiments done regarding this?
Well, according to the normal protocol we feed 1drop of milk in 250ml of DC water but in tank cultures I used to feed 5drops of milk in 5liters of dc water which means 1drop of milk in 1 litre of water…after observing the results I found that I was able to get more moinas in tanks as compared to bottle cultures where one drop milk was added to 250ml water.
Well first today I will try to take a look at the bottle in which the moina died.
I was feeding milk which was heated so I think that must have caused them to die due to loss of food. I will also need to discuss and find out about the Nitrates and nitrite test and check about colorimeter using mobile. As suggested by @jtd
We don’t know ammonia is present in what amount…
So, we @Sjuday2527@pratittodkar are trying to design an experiment to show the presence of ammonia in our cultures…
Is it possible that we can culture a specific breed of Bacteria that Moina can consume. Feeding them bacteria without the oils and fats from milk which later accumulate overtime as waste, can tremendously increase the quality of our cultures and there lifespan.
Yes buddy…what if we streak a loopful of milk on a milk agar or simply on a nutrient agar and get colonies of lactobacilli. Once we get colonies we can make a suspension of it and that we can try feeding the moinas. What say about this ?
